Distribution and molecular characterization of corynespora. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. Development of a lamp method for detecting the n75s mutant. First report of corynespora cassiicolaincited target spot. Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. Transformation of corynespora cassiicola by agrobacterium tumefaciens. Download pdf abstractexcerpt full text pdf contributor notes.
A new foliar disease, corynespora leaf spot, has been. Pathogenicity of corynespora cassiicola on different hosts in amazonas state. Inter simple sequence repeat issr and rdnaits sequence markers along. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. Corynespora leaf fall, caused by corynespora cassiicola, is one of the most important diseases in rubber hevea brasiliensis plantations. Corynespora leaf blight of cotton in brazil and its management. Corynespora cassiicola corynespora cassiicola berk.
The necrotrophic fungus corynespora cassiicola berk. General information about corynespora cassiicola coryca. The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Zhang, department of entomology and plant pathology, auburn university, al 36849. Characterization of the species corynespora cassiicola and. It is a member of the mitosporic ascomycota, a heterogeneous group of ascomycotic fungi whose common characteristic is the absence of a sexual state. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Wei is a cosmopolitan plant pathogen, especially abundant in the tropics. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot.
Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa. Crops that suffer the greatest yield loss due to the fungus include cowpea, cucumber, papaya, pepper, rubber, soybean and tomato. Wei from infected rubber leaves in sungai buloh two isolates and serdang two isolates were cultured on potato dextrose agar pda. A simple and efficient method to prepare 11chlorocyclopropyl21,2,4triazol1ylethan1one via nucleophilic substitution of 2chloro11chlorocyclopropylethanone and 1,2,4triazole is described. Sensitivity of corynespora cassiicola from soybean to. Pdf symptomatology, hosts, and pathogenicity of corynespora. Sobers the first report of corynespora cassiicola berk. Frontiers genomewide analysis of corynespora cassiicola. It is not yet known whether the same strain of this pathogen attacks both the crops. Cultural and reproductive variability of corynespora. Morphological features and inter simple sequence repeat issr polymorphism were employed to analyse 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in malaysia.
Transformation of corynespora cassiicola by agrobacterium. Morphological and inter simple sequence repeat issr. Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. The fungus causing target spot disease, corynespora cassiicola berk. Corynespora cassiicola is a species of fungus well known as a plant pathogen. The title compound is the key intermediate required for the synthesis of prothioconazole, a promising agricultural fungicide. Differentiation of corynespora cassiicola and cercospora. Naphthalenones and depsidones from a spongederived. Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. A boll rot of cotton caused by corynespora cassiicola in.
Keywords corynespora, leaf spot, chilli capsicum annuum l. Two new naphthalenones, corynenones a and b 1 and 2, and one new depsidone, corynesidone e 3, together with one known depsidone, corynesidone a 4 and two known diphenyl ethers, corynethers a 5 and b 6, were isolated from the spongederived fungus corynespora cassiicola xs20090i7. Two pathogens were isolated from tomatoes with severe chlorosis and necrosis in northwestern argentina. Their structures including absolute configurations were determined by. Corynespora cassiicola on chilli in west bengal, india.
Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has. This pathogen is common on a wide range of host plants 1,2,6,7,9,and is considered a predominantly tropical pathogen despite its common occurrence as a leaf, stem, and. In recent years, corynespora leaf blight is on the increase in cotton and has become an important disease in the state of mato grosso, brazil. Wei type species of the genus 100 described species found on diverse substrates. Symptomatology, hosts, and pathogenicity of corynespora cassiicola sintomatologia, hospedeiros e patogenicidade. Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. Corynespora cassiicola causing leaf spots on coleus barbatus corynespora cassiicola causing leaf spots on coleus barbatus fernandes, r. Detection of corynespora cassiicola in hevea rubber tree. Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis.
Pdf sensitivity of corynespora cassiicola from soybean. Pdf pathogenicity of corynespora cassiicola on different hosts in. Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer. Genomewide analysis of corynespora cassiicola leaf fall. Corynespora leaf spot or target spot is caused the fungus corynespora cassiicola, which causes leaf spots on cucumber, sweet potato, soybean, tomato and cotton. Corynespora cassiicola and stemphylium lycopersici. On cotton, it begins a small brickred spot which then leads to the formation of large 14 to 1in. Diversity of corynespora cassiicola isolates and changes. Structural analysis of cassiicolin, a hostselective. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. Genomic insight into pathogenicity of dematiaceous fungus. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. Corynespora cassiicola causing leaf spots on coleus.
A study was conducted to analyse the diversity among c. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. Chemical control of the soybean target spot corynespora. To the best of our knowledge, this study represents the first report of the sensitivity of previously unexposed greenhouse collected isolates of c. The organism was isolated and identified as corynespora cassiicola according to its. Phaeohyphomycosis caused by a plant pathogen, corynespora. Cookies are small text files that contain a string of characters and uniquely identifies a browser. Sensitivity loss by corynespora cassiicola, isolated from. However, the underlying factors that contribute to the.
It attacks several plant species including soybean. Development of a lamp method for detecting the n75s mutant in sdhiresistant corynespora cassiicola. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. First report of corynespora cassiicolaincited target spot on cotton in alabama. Conner, alabama cooperative extension system, auburn university, al 36849. Few studies referred to the formation of chlamydospores by c. All four isolates exhibited variability in colony colour and growth rates on artificial media pda, psa, rlea and wa. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385. Author links open overlay panel jiamei zhu a b 1 lingyan zhang a b 1 hong li a b yangyang gao a b wei mu a b feng. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and.
The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. Department of plant pathology, bidhan chandra krishi viswavidyalaya. Abstract the fungus corynespora cassiicola, causal agent of target spot in soybeans, can.
Soybean target leaf spot, caused by the fungus corynespora cassiicola, is controlled especially by leaf application of fungicides. It is widely used as a medicine against liver and digestion ailments in brazil. A new boll rot disease of cotton gossypium hirsutum l. Diagnostic features of corynespora cassiicola and its. On the inoculated stem there are sunken, pale greyish, elliptical lesions, measuring 1. In this study, iprodione ec 50 values ranged from 0. One of the major fungal plant diseases in the tropical and subtropical regions of the world is corynespora cassiicola. Most browsers are initially set up to accept cookies, since this is required by most website owners in order to access their sites. He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. They are sent to a computer by website operators or third parties. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. It has been reported in more than 70 host plants including economically important crops such as rubber tree, tomato, cucumber, cotton, soybean, tobacco, cocoa, cowpea,1. The objectives of the present investigation were to verify the genotypic and phenotypic variation between the.
A total of 618 isolates of corynespora leaf spot fungus corynespora cassiicola collected from 24 commercial cucumber greenhouses in 12 cities in ibaraki prefecture, japan, were tested for their sensitivity to boscalid. Frontiers compounds released by the biocontrol yeast. The objective of this study was to test the formation of chlamydospores by. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was.
The purpose of this research is to find the reliable means to determine the risk posed to agriculture in the pacific basins by the. Since that time, corynespora cassiicola has been identified as the cause of leaf spots on weeping fig ficus benjamina 3. Chase the first report of a corynespora leaf spot of a foliage plant was made in 1973 on zebra plant aphelandra squarrosa 4. Species in the genus induce corynespora leaf spot in bleeding hearts clerodendrum thomsoniae. Wei was greatly enhanced when the cultures were first scraped after 3 days growth and then grown for an additional 3 days in continuous light.
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